标题: Resistance of cyanobacteria Microcystis aeruginosa to erythromycin with multiple exposure

作者: Wu, YX (Wu, Yixiao); Wan, L (Wan, Liang); Zhang, WH (Zhang, Weihao); Ding, HJ (Ding, Huijun); Yang, WF (Yang, Wenfeng)

来源出版物: CHEMOSPHERE 卷: 249 文献号: 126147 DOI: 10.1016/j.chemosphere.2020.126147 出版年: JUN 2020

摘要: Here we report a set of experiments in which water blooming cyanobacteria Microcystis aeruginosa was repeatedly exposed to erythromycin. Growth inhibition increased with increasing erythromycin concentration (1-150 mu g/L) upon first exposure. Maximum inhibition rate (76.06%), occurred under 150 mu g/L erythromycin. Moreover, 96-h 50% effective concentration (EC50) was 22.97 mu g/L, indicating that the growth of M. aeruginosa was affected by erythromycin under common environmental concentrations. Photosynthesis was hindered by chlorophyll and photosystem II limitations. Malondialdehyde, reactive oxygen species, and superoxide dismutase contents increased significantly under certain concentrations of erythromycin, but superoxide dismutase was suppressed by 150 mu g/L erythromycin. Synthesis of intracellular and extracellular microcystins was promoted by 10-60 and by 20-60 mu g/L erythromycin, respectively, but both were inhibited by 100-150 mu g/L. Principal component analysis and Pearson's correlation revealed the accumulation of reactive oxygen species as the dominant mechanism of erythromycin toxicity to cells. M. aeruginosa repeatedly subjected to erythromycin exposure showed obvious resistance against the antibiotic, especially when treated twice with 60 mu g/L erythromycin. The 96-h EC50 was 81.29 mu g/L. As compared to the first exposure to erythromycin, photosynthetic and antioxidant activities increased, while growth inhibition and oxidation stress decreased upon multiple exposures. Production and release of microcystins were enhanced by repeated exposure to the antibiotic. Thus, erythromycin persistence in water should be examined, as repeated exposure may lead to serious environmental and human health hazards.

入藏号:  WOS:000527935600055

语言: English

文献类型: Article

作者关键词: Erythromycin; Microcystis aeruginosa; Antibiotics resistance; Microcystin; Oxidative stress

地址：[Wu, Yixiao; Wan, Liang; Yang, Wenfeng; Zhang, Weihao] Wuhan Univ, Sch Resource & Environm Sci, Key Lab Biomass Resource Chem & Environm Biotechn, Wuhan 430072, Peoples R China.

[Ding, Huijun] Jiangxi Prov Inst Water Sci, Jiangxi Prov Key Lab Water Resources & Environm P, Nanchang 330029, Jiangxi, Peoples R China.

通讯作者: Zhang, WH(通讯作者)， Wuhan Univ, Sch Resource & Environm Sci, Key Lab Biomass Resource Chem & Environm Biotechn, Wuhan 430072, Peoples R China.

电子邮件地址: zhangwh@whu.edu.cn

影响因子： 5.778