标题：Novel Layer-by-Layer Structured Nanofibrous Mats Coated by Protein Films for Dermal Regeneration作者：Xin, Shangjing; Li, Xueyong; Wang, Qun; Huang, Rong; Xu, Xiaoli; Lei,Zhanjun;Deng, Hongbing

来源出版物：JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 卷：10 期：5 页：803-810 DOI：10.1166/jbn.2014.1748出版年：MAY 2014

摘要：Layer-by-layer coating technique is effective in modifying the surface of nanofibrous mats, but overmuch film-coating makes the mats less porous to hardly suit the condition for tissue engineering. We developed novel nanofibrous mats layer-by-layer coated by silk fibroin and lysozyme on the cellulose electrospun template via electrostatic interaction. The film-coating assembled on the mats was not excessive because the charge of the proteins varied in the coating process due to different pH value. In addition, pure nature materials made the mats nontoxic, biodegradable and low-cost. The morphology and composition variation during layer-by-layer coating process was investigated and the results showed that the structure and thickness of film-coatings could be well-controlled. The antibacterial assay and in vitro cell experiments indicated that the mats could actively inhibit bacteria and exhibit excellent biocompatibility. In vivo implant assay further verified the mats cultured with human epidermal cells could promote wound healing and avoid wound infection. Therefore, these mats showed promising prospects when performed for dermal reconstruction.

入藏号：WOS:000327511400009

文献类型：Article

语种：English

作者关键词：Layer-by-Layer Coating Technique, Silk Fibroin, Lysozyme, Nanofibrous Mats, Dermal Reconstruction

扩展关键词：CHITOSAN NANOFIBERS; IN-VITRO; SCAFFOLDS; NANOPARTICLES; DELIVERY; FABRICATION; COMPOSITES; SILICATE; VIVO

通讯作者地址：Deng, Hongbing;Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

电子邮件地址：alphabeita@yahoo.com

地址：

[Xin, Shangjing; Deng, Hongbing] Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

[Xin, Shangjing; Huang, Rong; Deng, Hongbing] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.

[Li, Xueyong; Huang, Rong; Xu, Xiaoli; Lei, Zhanjun; Deng, Hongbing] Fourth Mil Med Univ, Dept Plast Surg, Tangdu Hosp, Xian 710038, Peoples R China.

[Wang, Qun] Iowa State Univ, Dept Chem & Biol Engn, Dept Civil, Ames, IA 50011 USA.

研究方向：Science & Technology - Other Topics; Materials Science

ISSN：1550-7033