标题：Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

作者：Zhao, Jiemin; Wang, Xiaoping; Kuang, Yanshen; Zhang, Yufeng; Shi,Xiaowen; Liu, Xingyun; Deng, Hongbing

来源出版物：RSC ADVANCES 卷：4 期：46 页：24369-24376 DOI：10.1039/c4ra02780a 出版年：2014

摘要：Alginate (ALG)-lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG-LZ beads via layer-by-layer (LBL) self-assembly technique. The mechanical properties and the enzymatic activity of these samples were studied by regulating the number of deposition bilayers and the composition of the outermost layer. The scanning electron microscopy images indicated that the resultant samples exhibited good sphericity and porosity. The Fourier transform infrared spectra results implied the presence of electrostatic interactions between ALG and LZ. The pore size distribution results revealed that the samples mainly possessed mesopores with radius in the range of 2-7 nm. In vitro LZ release test performed at different time intervals showed that LZ could be released from ALG-LZ beads and LBL film-coated beads. The amount of released LZ increased with extended time intervals.

入藏号：WOS:000338042500063

文献类型：Article

语种：English

扩展关键词：NANOFIBROUS MATS; PROTEIN DELIVERY; DRUG-DELIVERY; ALGINATE MATRICES; ORAL DELIVERY; CELL-CULTURE; CHITOSAN; SYSTEMS; MICROSPHERES; CELLULOSE

通讯作者地址：Deng, Hongbing;Wuhan Univ, Sch Resource & Environm Sci, Dept Environm Sci, Wuhan 430079, Peoples R China.

电子邮件地址：alphabeita@yahoo.com; hbdeng@whu.edu.cn

地址：

[Zhao, Jiemin; Shi, Xiaowen; Liu, Xingyun; Deng, Hongbing] Wuhan Univ, Sch Resource & Environm Sci, Dept Environm Sci, Wuhan 430079, Peoples R China.

[Zhao, Jiemin; Zhang, Yufeng] Wuhan Univ, Stomatol Hosp, Hubei MOST KLOS, Wuhan 430079, Peoples R China.

[Zhao, Jiemin; Zhang, Yufeng] Wuhan Univ, Hosp, KLOBME, Wuhan 430079, Peoples R China.

[Wang, Xiaoping] Fourth Mil Med Univ, Tangdu Hosp, Dept Thorac Surg, Xian 710038, Peoples R China.

[Kuang, Yanshen] Wuhan Univ, Zhongnan Hosp, Wuhan 430079, Peoples R China.

研究方向：Chemistry

ISSN：2046-2069