标题: Catechol Patterned Film Enables the Enzymatic Detection of Glucose with Cell Phone Imaging

作者: Wu, S (Wu, Si); Rzasa, JR (Rzasa, John R.); Kim, E (Kim, Eunkyoung); Zhao, ZL (Zhao, Zhiling); Li, JY (Li, Jinyang); Bentley, WE (Bentley, William E.); Payne, NN (Payne, Nadine N.); Shi, XW (Shi, Xiaowen); Payne, GF (Payne, Gregory F.)

来源出版物: ACS SUSTAINABLE CHEMISTRY & ENGINEERING 卷: 9 期: 44 页: 14836-14845 DOI: 10.1021/acssuschemeng.1c04896 出版年: NOV 8 2021

摘要: Biology provides an array of materials and mechanisms to create sustainable high-performance materials. Here, we prepared a polysaccharide film and patterned it with redox-active catechol moieties using a biomimetic oxidative grafting method. In this proof-of-concept study, we show that the film allows results from an enzymatic biosensing reaction to be reported through cell phone imaging for the detection of glucose in food and beverage products (glucose is a marker for high-fructose corn syrup; HFCS). This method uses glucose dehydrogenase (GDH) to confer molecular recognition, while the NADH product transfers its electrons to catechol moieties that are patterned onto a polysaccharide film. The "accumulation" of electrons by the catechol pattern can be detected because the oxidized quinone state is darker in color than the reduced catechol state. Using a commercially available cell phone imaging app, we demonstrate that HFCS-containing products can be distinguished from analogous products containing either sucrose or artificial sweeteners. Thus, we envision that this method could allow buyers or consumers to detect glucose on-site or in-home. In addition, this work further illustrates the potential of catechol-based materials for performing redox-based functions.

作者关键词: chitosan; catechols; redox; glucose dehydrogenase; high fructosecorn syrup (HFCS); cell phone imaging

地址: [Wu, Si; Shi, Xiaowen] Wuhan Univ, Hubei Biomass Resource Chem & Environm Biotechnol, Hubei Engn Ctr Nat Polymers Based Med Mat, Sch Resource & Environm Sci,Hubei Int Sci & Techn, Wuhan 430079, Peoples R China.

[Wu, Si] Wuhan Univ Sci & Technol, Coll Resources & Environm Engn, Wuhan 430081, Peoples R China.

[Rzasa, John R.; Kim, Eunkyoung; Zhao, Zhiling; Bentley, William E.; Payne, Gregory F.] Univ Maryland, Robert E Fischell Inst Biomed Devices, College Pk, MD 20742 USA.

[Rzasa, John R.; Li, Jinyang; Bentley, William E.] Univ Maryland, Fischell Dept Bioengn & Res, College Pk, MD 20742 USA.

[Kim, Eunkyoung; Zhao, Zhiling; Li, Jinyang; Bentley, William E.; Payne, Gregory F.] Univ Maryland, Inst Biosci & Biotechnol Res, College Pk, MD 20742 USA.

[Payne, Nadine N.] McCormick & Co Inc, Res & Dev, Baltimore, MD 21031 USA.

通讯作者地址: Shi, XW (通讯作者)，Wuhan Univ, Hubei Biomass Resource Chem & Environm Biotechnol, Hubei Engn Ctr Nat Polymers Based Med Mat, Sch Resource & Environm Sci,Hubei Int Sci & Techn, Wuhan 430079, Peoples R China.

Payne, GF (通讯作者)，Univ Maryland, Robert E Fischell Inst Biomed Devices, College Pk, MD 20742 USA.

Payne, GF (通讯作者)，Univ Maryland, Inst Biosci & Biotechnol Res, College Pk, MD 20742 USA.

电子邮件地址: shixw@whu.edu.cn; gpayne@umd.edu

影响因子：8.198