标题：Nanofibrous Mats Layer-by-Layer Assembled by HTCC/Layered Silicate Composites with In Vitro Antitumor Activity Against SMMC-7721 Cells作者：Huang, Rong; Zhou, Xue; Liu, Xinqin; Zhang, Qi; Jin, Huan'guang; Shi,Xiaowen;Luo, Wenjing;Deng, Hongbing

来源出版物：JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 卷：10 期：3 页：485-499 DOI：10.1166/jbn.2014.1716 出版年：MAR 2014

摘要：Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique. Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm. Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template. MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells. Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples. The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.

入藏号：WOS:000327497900012

文献类型：Article

语种：English

作者关键词：Organic Rectorite, Polymer Nanocomposites, Layer-by-layer, Antitumor Activity

扩展关键词：BACTERIAL-INHIBITION ACTIVITY; ANTIMICROBIAL ACTIVITY; QUATERNIZED CHITOSAN; FILMS; NANOCOMPOSITE; SCAFFOLDS; IMMOBILIZATION; FABRICATION; ALGINATE; DELIVERY

通讯作者地址：Luo, Wenjing;Fourth Mil Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Xian 710032, Peoples R China.

Deng,Hongbin;Wuhan Univ,Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

电子邮件地址：alphabeita@yahoo.com; luowenj@fmmu.edu.cn

地址：

[Huang, Rong; Shi, Xiaowen; Deng, Hongbing] Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

[Huang, Rong; Zhang, Qi; Jin, Huan'guang; Deng, Hongbing] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.

[Huang, Rong; Zhang, Qi; Jin, Huan'guang; Deng, Hongbing] Huazhong Agr Univ, MOE Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China.

[Zhou, Xue] Huazhong Univ Sci & Technol, Sch Publ Hlth, Dept Occupat & Environm Hlth, Tongji Med Coll, Wuhan 430030, Peoples R China.

[Liu, Xinqin; Luo, Wenjing] Fourth Mil Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Xian 710032, Peoples R China.

研究方向：Science & Technology - Other Topics; Materials Science

ISSN：1550-7033