标题: Influencing factors of bioaugmentation treatment of PAH-contaminated soils in slurry bioreactors

作者: Bao, SY (Bao, Shiyu); Zhao, LK (Zhao, Likun); Liu, YW (Liu, Yuanwen); Yang, LW (Yang, Lewei); Liu, P (Liu, Peng); Chen, CQ (Chen, Chaoqi); Mao, XH (Mao, Xuhui)

来源出版物: JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING  卷: 11  期: 3  文献号: 109893  DOI: 10.1016/j.jece.2023.109893  出版年: JUN 2023  

摘要: Polycyclic aromatic hydrocarbons (PAHs) are a group of contaminants with low bioavailability and biodegradability. As a result, removing PAHs from contaminated soils is often inefficient. In this study, soils spiked with fluoranthene and pyrene were inoculated with Aeromonas sp. BCP-3, a newly isolated strain from coke-plant sludge, and treated in slurry bioreactors. The removal of PAHs was significantly affected by aeration rate, slurry water-soil mass ratio, stirring rate, temperature, and the concentration of Tween-80. Such effects were attributed to changes in BCP-3 growth, PAHs solid-liquid mass transfer, and slurry particle size. Notably, under optimal conditions (i.e., aeration rate of 1.5 L/min, temperature of 30 degrees C, stirring rate of 200 rpm, water-soil mass ratio of 1.5:1, and Tween-80 concentration of 1.3 %), 96.3 % and 97.2 % of initially spiked fluoranthene and pyrene were removed within 12 days, respectively. A PAH-contaminated soil was collected from a local coking plant and treated under the same conditions, except for the Tween-80 concentration. For the real soil, the Tween-80 concentration was 4 % to ensure a significant proportion of PAHs released into the liquid phase. Twenty-one days after inoculation of BCP-3, the removal efficiencies of low molecular weight PAHs and high molecular weight PAHs were 82.2 % and 51.8 %, respectively. The removal efficiencies of PAHs were significantly higher than the slurry without BCP-3 or HgCl2-sterilized slurry. BCP-3 improved the bioavailability and biodegradability of PAHs, resulting in effective PAH removal in slurry bioreactors. Overall, successful bioaugmentation was achieved with the addition of Tween-80 and the optimization of operating conditions.

作者关键词: Polycyclic aromatic hydrocarbons; Slurry bioreactors; Bioremediation; Bioaugmentation; Influencing factors

KeyWords Plus: POLYCYCLIC AROMATIC-HYDROCARBONS; ORGANIC POLLUTANTS; BIODEGRADATION; BIOREMEDIATION; REMOVAL; DEGRADATION; SURFACTANT; PYRENE; PHENANTHRENE; REMEDIATION

地址: [Bao, Shiyu; Zhao, Likun; Mao, Xuhui] Wuhan Univ, Sch Resource & Environm Sci, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

[Liu, Yuanwen; Yang, Lewei; Liu, Peng] Beijing Construct Engn Grp Environm Remediat Co Lt, Beijing 10001, Peoples R China.

[Chen, Chaoqi] Wuhan Univ, Sch Resource & Environm Sci, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430079, Peoples R China.

通讯作者地址: Mao, XH (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

Chen, CQ (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430079, Peoples R China.

电子邮件地址: chenchaoqi@whu.edu.cn; clab@whu.edu.cn

影响因子：7.77