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赵忠涛(硕士生)、施晓文的论文在COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS刊出
发布时间:2024-10-21     发布者:易真         审核者:任福     浏览次数:

标题: Sodium dodecyl sulfate assisted electric field treatment for deproteinization of chitosan

作者: Zhao, ZT (Zhao, Zhongtao); Qiu, YH (Qiu, Yuhuan); Zhang, JX (Zhang, Jingxian); Xu, YC (Xu, Yuncheng); Deng, HB (Deng, Hongbing); Du, YM (Du, Yumin); Shi, XW (Shi, Xiaowen)

来源出版物: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS : 703 文献号: 135187 DOI: 10.1016/j.colsurfa.2024.135187 Published Date: 2024 NOV 20 子辑: 1

摘要: Chitosan with a low protein residue is required as it is widely used in the field of biomedicine. Herein, we proposed a novel method for deproteinizing industrial grade chitosan via electric field treatment in a medium containing sodium dodecyl sulfate (SDS). The effects of electric field intensity, SDS concentration, and electrophoresis time on the protein removal efficiency were optimized by orthogonal experimental design and model building. Chitosan with an ultra-low protein residue (0.151 wt%) can be obtained at the voltage of 300 V, the SDS concentration of 1 wt%, and the electrophoresis time of 2 h. The strong binding between SDS and residue protein renders the conjugate with negative charges, which can migrate under the electric field. In addition, the electric field deproteinization process reduces the heavy metal content of chitosan slightly, while keeping the chemical structure of chitosan unchanged. The methodology provided in this work suggests the great potential of electric field treatment in removing residue protein in chitosan.

作者关键词: Chitosan; Deproteinization; Orthogonal experiment; Sodium dodecyl sulfate; Electrophoresis

地址: [Zhao, Zhongtao; Qiu, Yuhuan; Zhang, Jingxian; Xu, Yuncheng; Deng, Hongbing; Du, Yumin; Shi, Xiaowen] Wuhan Univ, Hubei Engn Ctr Nat Polymers Based Med Mat, Sch Resource & Environm Sci, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430079, Peoples R China.

[Zhao, Zhongtao] Hubei Fisheries Sci Res Inst, Wuhan 430079, Hubei, Peoples R China.

通讯作者地址: Shi, XW (通讯作者)Wuhan Univ, Hubei Engn Ctr Nat Polymers Based Med Mat, Sch Resource & Environm Sci, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430079, Peoples R China.

电子邮件地址: shixw@whu.edu.cn

影响因子:4.9